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Rapid detection of viable bacteria system and method

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posted on 2022-01-16, 00:00 authored by Hsueh-Chia ChangHsueh-Chia Chang, Sachidevi Puttaswamy, Shramik Sengupta
An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample.

History

Patent Number

US 10273522 B2

Other Application

14/099,110

Inventor

Shramik Sengupta Sachidevi Puttaswamy Hsueh-Chia Chang

Inventor from Local Institution

Hsueh-Chia Chang

Assignee

University of Missouri (Columbia, MO) University of Notre Dame du Lac

Date Modified

2022-02-04

Language

  • English

Claims

18

Prior Publication Number

US 2014/0162310 A1

Cooperative Patent Classification Codes

G01N 33/5438 (20130101); C12Q 1/06 (20130101); C12Q 1/04 (20130101); B01L 3/502715 (20130101)

Contributor

Hsueh-Chia Chang

International Patent Classification Codes

G01N 33/50 (20060101); C12Q 1/04 (20060101); C12Q 1/06 (20060101); G01N 33/543 (20060101); B01L 3/00 (20060101)

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