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Rapid detection of viable bacteria system and method

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posted on 2017-06-22, 00:00 authored by Hsueh-Chia ChangHsueh-Chia Chang, Shramik Sengupta
An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample.

History

Publisher

U.S. Patent and Trademark Office

Date Modified

2017-06-22

Contributor

Hsueh-Chia Chang

Language

  • English

Patent Number

US 8635028 B2

Other Application

12/896,188

Inventor

Shramik Sengupta Hsueh-Chia Chang

Inventor from Local Institution

Hsueh-Chia Chang

Assignee

The Curators of the University of Missouri (Columbia, MO) University of Notre Dame du Lac

Claims

18

Prior Publication Number

US 20110081676 A1

Cooperative Patent Classification Codes

C12Q 1/04 (20130101); G01N 33/5438 (20130101); C12Q 1/06 (20130101); B01L 3/502715 (20130101)

International Patent Classification Codes

G01N 33/50 (20060101)

US Patent Classification Codes

702/19

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