System for capillary electrophoresis for peptide and protein analysis

A method of coating the inside wall of a capillary with a polymeric material for capillary electrophoresis is disclosed. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.