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Investigations of proteins important for antibiotic resistance

thesis
posted on 2005-04-22, 00:00 authored by Yuridia Caire
The objective of my first project was to investigate the interaction of a class D beta-lactamase with different Beta-lactams to explore if this family of enzymes is diffusion-limited or not. This project required isolation and purification of the OXA-10 beta-lactamase overexpressed in E. coli BL21 and the determination of kcat, Km, and Vmax parameters for different beta-lactam antibiotics at different viscosities of the reaction buffer. The other project that I worked on required the cloning of blaI gene into an expression vector, overexpression of BlaI in E. coli BL21, purification of this protein. This protein is a gene repressor, believed to be involved in inducible expression of beta-lactamases in S. aureus.

History

Date Modified

2017-06-05

Research Director(s)

Dr. Paul Huber

Committee Members

Dr. Paul Helquist Dr. Bradley Smith Dr. Paul Huber

Degree

  • Master of Science

Degree Level

  • Master's Thesis

Language

  • English

Alternate Identifier

etd-04222005-100834

Publisher

University of Notre Dame

Program Name

  • Chemistry and Biochemistry

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