The Proteomic, Transcriptomic, and Metabolomic Analyses of Developing Xenopus laevis Embryos

Xenopus laevis has proven itself as a powerful and indispensable model organism in the field of developmental biology. However, it has begun to spread into other fields of research, one of which being analytical chemistry and quantitative proteomics. Beginning with the largest developmental proteome dataset of any organism at that time, Xenopus laevis has since been utilized in countless analytical experiments, ranging from single cell proteomics and metabolomics, protein extraction optimization, and phosphoproteomics. In this work, I continued integrating numerous analytical techniques while investigating various developmental aspects of Xenopus embryology. This has resulted in furthering single cell proteomic sensitivity, whole embryo metabolite imaging, N-­glycoproteome quantitation, conduction system analysis of the developing heart, and the integration of proteomics and transcriptomics investigating neural cell lineage commitment.