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  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large imaging depth, but also the strengths of FLIM including the ability to discriminate different fluorophores with similar emission spectra. In FLIM, the frequency-domain (FD) method, which relies on periodic intensity-modulated excitation light and i…

    Date Created:
    2015-10-22
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, conventional fluorescence microscopes cannot satisfy these needs due to physical limits on resolution, speed, information, and depth. In this work, we aim to overcome these limits using novel super-resolution microscopy and high-speed quantitative…

    Date Created:
    2018-04-09
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy (MPM) is a widely used in vivo imaging technique in biological and medical applications. In the case of two-photon excitation fluorescence, two lower-energy photons excite a fluorophore which in turn emits a single higher-energy photon, whose rate depends quadratically on the excitation intensity, thus enabling higher resolution with narrower point spread function (PSF) than conventional one-photon fluorescence microscopy. Fluorescence lifetime imaging microscopy (FLIM)…

    Date Created:
    2016-04-04
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform with super-sensitivity and super-resolution capabilities to quantitatively obtain this information at the intracellular level. First, we present a simple MPM oxygen imaging probe compatible with aqueous biological media based on water-soluble rut…

    Date Created:
    2017-03-30
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high cost. In this research, we propose and demonstrate a new kind of super-resolution fluorescence microscopy that can be easily implemented and requires neither additional hardware nor complex post-processing. The microscopy is based on the principle o…

    Date Created:
    2017-10-19
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang, Scott S. Howard, Cody J. Smith
    Abstract:

    Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment. Other than a traditional confocal or two-photon microscope, this approac…

    Date Created:
    2019-01-09
    Resource Type
    Software
  • Author:
    Raj K Jana
    Advisory Committee:
    Dr. Gregory Snider, Dr. Anthony Hoffman, Dr. Scott S. Howard, Dr. Debdeep Jena
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2015-04-07
  • Author:
    Chad A. Stephenson
    Advisory Committee:
    Doug Hall, Mark Wistey, Greg Snider, Debdeep Jena
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2015-04-03
  • Author:
    Farzad Talebi
    Advisory Committee:
    Dr. Thomas Fuja, Dr. Nicholas Laneman, Dr. Thomas Pratt, Dr. Martin Haenggi
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2015-03-30
  • Author:
    Cheng-Zong Bai
    Advisory Committee:
    Bertrand Hochwald, Ken Sauer, Yih-Fang Huang, Vijay Gupta
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2015-04-09
  • Author:
    Lai Wei
    Advisory Committee:
    Thomas E. Fuja, Bertrand Hochwald, J. Nicholas Laneman, Yih-Fang Huang, David G. M. Mitchell, Daniel J. Costello, Jr.
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2014-09-09
  • Author:
    Jie Liu
    Advisory Committee:
    Panos Antsaklis, Vijay Gupta, Ken Sauer, Matias Negrete-Pincetic
    Degree Area:
    Electrical Engineering
    Degree:
    PhD
    Defense Date:
    2015-04-07