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  • Author(s):
    David Severson, Anastasia N. Naumenko, Vladimir A. Timoshevskiy, Nicholas A. Kinney, Alina A. Kokhanenko, Becky S. deBruyn, Diane D. Lovin, Vladimir N. Stegniy, Igor V. Sharakhov, Maria V. Sharakhova
    Abstract:

    The genome assembly of southern house mosquito Cx. quinquefasciatus is represented by a high number of supercontigs with no order or orientation on the chromosomes. Although cytogenetic maps for the polytene chromosomes of this mosquito have been developed, their utilization for the genome mapping remains difficult because of the low number of high-quality spreads in chromosome preparations. Therefore, a simple and robust mitotic-chromosome-based approach for the genome mapping of Cx. quinque…

  • Author(s):
    David Severson, Susanta K. Behura, Consuelo Gomez-Machorro, Brent W. Harker, Becky deBruyn, Diane D. Lovin, Ryan R. Hemme, Akio Mori, Jeanne Romero-Severson
    Abstract:

    Dengue virus is primarily transmitted by Aedes aegypti mosquitoes. Control of the vector mosquito is the major practice to prevent dengue. However, it is not well known how the virus can infect some mosquito strains but fail to do so with other refractory strains. To address that question, we conducted whole genome microarray based gene expression studies between susceptible and refractory strains of A. aegypti to identify gene expression patterns following challenge with dengue virus. Our an…