Harper Cancer Research Institute
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- Record Visibility:
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2
Article
- Author(s):
- Eldin Jasarević, Jie Ning, Ashley N Daniel, Rachel A Menegaz, Jeffrey J Johnson, M Sharon Stack, Matthew J Ravosa
- Abstract:
In contrast to experimental evidence regarding the postorbital bar, postorbital septum, and browridge, there is exceedingly little evidence regarding the load-bearing nature of soft-tissue structures of the mammalian circumorbital region. This hinders our understanding of pronounced transformations during primate origins, in which euprimates evolved a postorbital bar from an ancestor with the primitive mammalian condition where only soft tissues spanned the lateral orbital margin between fron…
- Date Published:
- 2010-04-01
- Record Visibility:
- Public
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- Author(s):
- Matthew J Ravosa, Elisabeth K López, Rachel A Menegaz, Stuart R Stock, M Sharon Stack, Mark W Hamrick
- Abstract:
Knockout mice lacking myostatin (Mstn), a negative regulator of the growth of skeletal muscle, develop significant increases in the relative mass of masticatory muscles as well as the ability to generate higher maximal muscle forces. Wild-type and Mstn-deficient mice were compared to investigate the postnatal influence of elevated masticatory loads due to increased jaw-adductor and bite forces on the biomineralization of mandibular articular and cortical bone, the internal structure of the ja…
- Date Published:
- 2008-09-01
- Record Visibility:
- Public
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- Author(s):
- Michelle M Woo, Clara M Salamanca, Jaime Symowicz, M Sharon Stack, Dianne M Miller, Peter C Leung, C Blake Gilks, Nelly Auersperg
- Abstract:
Objective: Serous borderline ovarian tumors (SBOT) are slow growing, noninvasive ovarian epithelial neoplasms, which tend to recur as low-grade invasive carcinomas (LGC) with a much worse prognosis. We investigated the molecular basis of this progression. Methods: We established cultures of three SBOTs and one LGC from tumor biopsies, and inactivated p53, Rb and PP2A in the cells with SV40 large T (LT) and small T (ST) antigen. They were examined for cadherins by immunofluorescence and immun…
- Date Published:
- 2008-10-01
- Record Visibility:
- Public
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- Author(s):
- Zonggao Shi, Jeffrey J Johnson, Rong Jiang, Yueying Liu, M Sharon Stack
- Abstract:
With the aim to identify microRNAs that may contribute to oral squamous cell carcinoma (OSCC) progression, we compared the microRNA expression profiles of two related cell lines that form tumors with differential aggressiveness. A panel of 28 microRNAs was found to be more than 1.5-fold altered, among which miR-146a was the most significantly changed (-4.6-fold). Loss of miR-146a expression was validated in human high-grade tumors, while normal oral mucosa retained expression, using fluoresce…
- Date Published:
- 2015-09
- Record Visibility:
- Public
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- Author(s):
- Zonggao Shi, Jeff J Johnson, M Sharon Stack
- Abstract:
The technique of nucleic acid in situ hybridization is an effective method for identifying the existence and abundance of nucleic acids in tissue sections or cytological preparations. Such a method has the advantage of keeping morphological relationships intact while identifying changes at the molecular level. As a noncoding regulatory RNA, microRNA has been found to intricately control many physiological and pathological conditions. We provide here a representative fluorescence in situ hybri…
- Date Published:
- 2013-07-02
- Record Visibility:
- Public
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- Author(s):
- Y Liu, S M Stack, S S Lakka, A J Khan, D T Woodley, J S Rao, C N Rao
- Abstract:
Human tissue factor pathway inhibitor-2 (TFPI-2)/matrix-associated serine protease inhibitor (MSPI), a Kunitz-type serine protease inhibitor, inhibits plasmin, trypsin, chymotrypsin, plasma kallikrein, cathepsin G, and factor VIIa-tissue factor complex. The mature protein has a molecular mass of 32-33 kDa, but exists in vivo as two smaller, underglycosylated species of 31 and 27 kDa. TFPI-2/MSPI triplet is synthesized and secreted by a variety of cell types that include epithelial, endothelia…
- Date Published:
- 1999-10-01
- Record Visibility:
- Public
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- Author(s):
- Jochen H Lorch, Jodi Klessner, J Ken Park, Spiro Getsios, Yvonne L Wu, M Sharon Stack, Kathleen J Green
- Abstract:
The epidermal growth factor receptor (EGFR) has been proposed as a key modulator of cadherin-containing intercellular junctions, particularly in tumors that overexpress this tyrosine kinase. Here the EGFR tyrosine kinase inhibitor PKI166 and EGFR blocking antibody C225, both of which are used clinically to treat head and neck cancers, were used to determine the effects of EGFR inhibition on intercellular junction assembly and adhesion in oral squamous cell carcinoma cells. EGFR inhibition res…
- Date Published:
- 2004-08-27
- Record Visibility:
- Public
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- Author(s):
- Reema Zeineldin, Martina Rosenberg, Dominic Ortega, Christian Buhr, Miquella G Chavez, M Sharon Stack, Donna F Kusewitt, Laurie G Hudson
- Abstract:
Overexpression of the epidermal growth factor (EGF) receptor occurs frequently in ovarian cancer and is associated with poor patient prognosis. A constitutively active mutant EGF receptor termed variant III (EGFRvIII) has been detected at a high frequency in many human tumors, including those of the ovary. To identify the consequences of EGFRvIII expression in ovarian tumor cells, we introduced EGFRvIII into the epithelial ovarian cancer cell line (OVCA 433). The EGFRvIII-transfected cells di…
- Date Published:
- 2006-11-01
- Record Visibility:
- Public
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- Author(s):
- S Ghosh, R Brown, J C Jones, S M Ellerbroek, M S Stack
- Abstract:
Expression of urinary-type plasminogen activator (uPA) and its receptor (uPAR) is correlated with matrix proteolysis, cell adhesion, motility, and invasion. To evaluate the functional link between adhesion and proteolysis in gingival keratinocytes (pp126), cells were treated with immobilized integrin antibodies to induce integrin clustering. Clustering of alpha(3) and beta(1) integrin subunits, but not alpha(2), alpha(5), alpha(6), or beta(4), enhanced uPA secretion. Bead-immobilized laminin-…
- Date Published:
- 2000-02-02
- Record Visibility:
- Public
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- Author(s):
- L E Goldfinger, M S Stack, J C Jones
- Abstract:
The laminin-5 component of the extracellular matrices of certain cultured cells such as the rat epithelial cell line 804G and the human breast epithelial cell MCF-10A is capable of nucleating assembly of cell- matrix adhesive devices called hemidesmosomes when other cells are plated upon them. These matrices also impede cell motility. In contrast, cells plated onto the laminin-5-rich matrices of pp126 epithelial cells fail to assemble hemidesmosomes and are motile. To understand these contrad…
- Date Published:
- 1998-04-06
- Record Visibility:
- Public
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- Author(s):
- Thuy-Vy Do, Jay C Symowicz, David M Berman, Lance A Liotta, Emanuel F Petricoin 3rd, M Sharon Stack, David A Fishman
- Abstract:
Epithelial ovarian cancer (EOC) is asymptomatic at early stages and is often diagnosed late when tumor cells are highly metastatic. Lysophosphatidic acid (LPA) has been implicated in ovarian oncogenesis as levels of this lipid are elevated in patient ascites and plasma. Because the underlying mechanism governing LPA regulation of matrix metalloproteinase-2 (MMP-2) activation remains undefined, we investigated the relationship between LPA-induced changes in actin microfilament organization and…
- Date Published:
- 2007-02-01
- Record Visibility:
- Public