Notre Dame Research

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  Article

  Methods for the visualization and analysis of extracellular matrix protein structure and degradation
  

  Author(s):

  Annemarie K. Leonard, Elizabeth A. Loughran, Yuliya Klymenko, Yueying Liu, Oleg Kim, Marwa Asem, Kevin McAbee, Matthew J. Ravosa, M. Sharon Stack

  Abstract:

  This chapter highlights methods for visualization and analysis of extracellular matrix (ECM) proteins, with particular emphasis on collagen type I, the most abundant protein in mammals. Protocols described range from advanced imaging of complex in vivo matrices to simple biochemical analysis of individual ECM proteins. The first section of this chapter describes common methods to image ECM components and includes protocols for second harmonic generation, scanning electron microscopy, and seve…

  Date Published:

  2018

  Record Visibility:

  Public