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  • Author(s):
    Genevieve D. Vigil, Lina Cao, Aamir A. Khan, David Benirschke, Tahsin Ahmed, Patrick Fay, Scott S. Howard
    Abstract:

    Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM at high excitation powers. In this Letter, we present an analytical theoretical description of this error and present a method for compensating for this error in order to extract correct lifetime measurements in the limit of fluorophore satura…

    Date Published:
    2016-12
    Record Visibility:
    Public