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  Article

  Saturation-compensated measurements for fluorescence lifetime imaging microscopy
  

  Author(s):

  Genevieve D. Vigil, Lina Cao, Aamir A. Khan, David Benirschke, Tahsin Ahmed, Patrick Fay, Scott S. Howard

  Abstract:

  Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM at high excitation powers. In this Letter, we present an analytical theoretical description of this error and present a method for compensating for this error in order to extract correct lifetime measurements in the limit of fluorophore satura…

  Date Published:

  2016-12

  Record Visibility:

  Public