Search CurateND

Search criteria:

Clear all

List of files deposited in CurateND that match your search criteria

  • Creator(s):
    Varun Mannam
    Description:

    We created a small dataset to validate the lifetime denoising using the trained Noise2Noise-NBN model - the Fluorescence Microscopy Lifetime Denoising (FMLD) dataset - that is dedicated to perform the lifetime denoising from the noisy (raw) lifetime images.

    The dataset consists of 50 real fluorescence microscopy images obtained with custom-built two-photon microscope and representative fixed BPAE cell (F36924) samples. We use all 50 images for testing/inference. These images are captured wi…

    Date Created:
    2020-01-01
    Record Visibility:
    Public
  • Creator(s):
    Varun Mannam, Yide Zhang, Yinhao Zhu, Scott Howard
    Description:

    We created a dataset - the Fluorescence Microscopy Denoising (FMD) dataset - that is dedicated to Poisson-Gaussian denoising. The dataset consists of 12,000 real fluorescence microscopy images obtained with commercial confocal, two-photon, and wide-field microscopes and representative biological samples such as BPAE cells, zebrafish, and mouse brain tissues. We use image averaging to effectively obtain ground truth images and 60,000 noisy images with different noise levels.

    Date Created:
    2019-04-21
    Record Visibility:
    Public
  • Author(s):
    Christianos Burlotos
    Abstract:

    The residential construction industry in developing nations is often plagued by numerous constraining systems, including underdeveloped financial markets, ineffective governing bodies, and unregulated material and labor markets, which restrict safe and disaster-resilient housing to the wealthy minority. The majority are left in nonengineered, informally constructed homes, which, in countries with high exposure to hydro-meteorological and/or seismic hazards, can prove deadly. The thesis presen…

    Record Visibility:
    Public
  • Creator(s):
    Christianos Burlotos
    Description:

    This multilayered spreadsheet is used to assist aspiring homeowners design their home from a pre-set list of options for floorplans, finishes, fixtures, and basic services. It also rapidly calculates an estimated total cost for the structure as well as a corresponding monthly payment amount. This allows the design process to be performed iteratively, giving families autonomy in the design of their own home, the chance to consider a variety of options and combinations, and the immediate knowle…

    Date Created:
    2020-07-20
    Record Visibility:
    Public
  • Creator(s):
    Christianos Burlotos
    Description:

    This tool calculates the design loads on a reinforced concrete special moment frame with a concrete slab roof, determines force and moment demands through an approximate portal frame analysis, assesses load combinations, evaluates the capacity of members compared to the demands, and approximates the cost of needed construction materials. It was produced by MSCE student Christianos Burlotos in order to parametrically assess frames to determine the best-fit frame for single-family homes in Leog…

    Date Created:
    2020-07-20
    Record Visibility:
    Public
  • Creator(s):
    Christianos Burlotos
    Description:

    This tool calculates the design loads on a reinforced concrete special moment frame with a corrugated galvanized iron roof, determines force and moment demands through an approximate portal frame analysis, assesses load combinations, evaluates the capacity of members compared to the demands, and approximates the cost of needed construction materials. It was produced by MSCE student Christianos Burlotos in order to parametrically assess frames to determine the best-fit frame for single-family …

    Date Created:
    2020-07-20
    Record Visibility:
    Public
  • Author(s):
    Patrick Calderon, Kathleen Kollman, Mehak Siddiquei, Shuyuan Shen
    Abstract:

    Various migration-related controversies in the United States and the 2015 migrant crisis in Europe have raised questions about destination countries’ treatment of migrants. This report, produced in collaboration with the department of Migration and Refugee Services of the United States Conference of Catholic Bishops, considers the post-arrival side of immigration enforcement in the United States, Greece, and Germany through a normative lens. It asks: What does it mean for immigration enfo…

    Date Created:
    2019-02-01
    Record Visibility:
    Public
    Resource Type
    Report
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large imaging depth, but also the strengths of FLIM including the ability to discriminate different fluorophores with similar emission spectra. In FLIM, the frequency-domain (FD) method, which relies on periodic intensity-modulated excitation light and i…

    Date Created:
    2015-10-22
    Record Visibility:
    Public
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, conventional fluorescence microscopes cannot satisfy these needs due to physical limits on resolution, speed, information, and depth. In this work, we aim to overcome these limits using novel super-resolution microscopy and high-speed quantitative…

    Date Created:
    2018-04-09
    Record Visibility:
    Public
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy (MPM) is a widely used in vivo imaging technique in biological and medical applications. In the case of two-photon excitation fluorescence, two lower-energy photons excite a fluorophore which in turn emits a single higher-energy photon, whose rate depends quadratically on the excitation intensity, thus enabling higher resolution with narrower point spread function (PSF) than conventional one-photon fluorescence microscopy. Fluorescence lifetime imaging microscopy (FLIM)…

    Date Created:
    2016-04-04
    Record Visibility:
    Public
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform with super-sensitivity and super-resolution capabilities to quantitatively obtain this information at the intracellular level. First, we present a simple MPM oxygen imaging probe compatible with aqueous biological media based on water-soluble rut…

    Date Created:
    2017-03-30
    Record Visibility:
    Public
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high cost. In this research, we propose and demonstrate a new kind of super-resolution fluorescence microscopy that can be easily implemented and requires neither additional hardware nor complex post-processing. The microscopy is based on the principle o…

    Date Created:
    2017-10-19
    Record Visibility:
    Public
    Resource Type
    Presentation