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  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large imaging depth, but also the strengths of FLIM including the ability to discriminate different fluorophores with similar emission spectra. In FLIM, the frequency-domain (FD) method, which relies on periodic intensity-modulated excitation light and i…

    Date Created:
    2015-10-22
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, conventional fluorescence microscopes cannot satisfy these needs due to physical limits on resolution, speed, information, and depth. In this work, we aim to overcome these limits using novel super-resolution microscopy and high-speed quantitative…

    Date Created:
    2018-04-09
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy (MPM) is a widely used in vivo imaging technique in biological and medical applications. In the case of two-photon excitation fluorescence, two lower-energy photons excite a fluorophore which in turn emits a single higher-energy photon, whose rate depends quadratically on the excitation intensity, thus enabling higher resolution with narrower point spread function (PSF) than conventional one-photon fluorescence microscopy. Fluorescence lifetime imaging microscopy (FLIM)…

    Date Created:
    2016-04-04
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform with super-sensitivity and super-resolution capabilities to quantitatively obtain this information at the intracellular level. First, we present a simple MPM oxygen imaging probe compatible with aqueous biological media based on water-soluble rut…

    Date Created:
    2017-03-30
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high cost. In this research, we propose and demonstrate a new kind of super-resolution fluorescence microscopy that can be easily implemented and requires neither additional hardware nor complex post-processing. The microscopy is based on the principle o…

    Date Created:
    2017-10-19
    Resource Type
    Presentation
  • Author(s):
    Tianze Peng, David Richter
    Abstract:

    Sea-spray droplets ejected into the air-sea boundary layer take part in a series of complex transport processes. To model the air-sea exchange of heat under high-wind conditions, it is important yet challenging to understand influences of evaporative droplets in the atmospheric boundary layer. We implement a high-resolution Eulerian-Lagrangian algorithm with varied droplets laden in a turbulent open-channel flow to reveal the dynamic and thermodynamic characteristics of droplets. Our past num…

    Date Created:
    2017-12-14
    Resource Type
    Presentation