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  • Author(s):
    Elizabeth A. Loughran, Ryan C. Phan, Annemarie K. Leonard, Laura Tarwater, Marwa Asem, Yueying Liu, Jing Yang, Yuliya Klymenko, Jeff Johnson, Zonggao Shi, Tyvette S. Hilliard, Marielle Blumenthaler, Matthew Leevy, Matthew J. Ravosa, M. Sharon Stack
    Abstract:

    Ovarian cancer is the fifth leading cause of cancer deaths in U.S. women and the deadliest gynecologic malignancy. This lethality is largely due to the fact that most cases are diagnosed at metastatic stages of the disease when the prognosis is poor. Epidemiologic studies consistently demonstrate that parous women have a reduced risk of developing ovarian cancer, with a greater number of births affording greater protection; however little is known about the impact of parity on ovarian cancer …

    Date Published:
    2017
  • Author(s):
    Annemarie K. Leonard, Elizabeth A. Loughran, Yuliya Klymenko, Yueying Liu, Oleg Kim, Marwa Asem, Kevin McAbee, Matthew J. Ravosa, M. Sharon Stack
    Abstract:

    This chapter highlights methods for visualization and analysis of extracellular matrix (ECM) proteins, with particular emphasis on collagen type I, the most abundant protein in mammals. Protocols described range from advanced imaging of complex in vivo matrices to simple biochemical analysis of individual ECM proteins. The first section of this chapter describes common methods to image ECM components and includes protocols for second harmonic generation, scanning electron microscopy, and seve…

    Date Published:
    2018
  • Author(s):
    Elizabeth A. Loughran, Annemarie K. Leonard, Tyvette S. Hilliard, Ryan C. Phan, Madeleine G. Yemc, Elizabeth Harper, Emma Sheedy, Yuliya Klymenko, Marwa Asem, Yueying Liu, Jing Yang, Jeff Johnson, Laura Tarwater, Zonggao Shi, Matthew Leevy, Matthew J. Ravosa, M. Sharon Stack
    Abstract:

    Ovarian cancer, the most deadly gynecological malignancy in U.S. women, metastasizes uniquely, spreading through the peritoneal cavity and often generating widespread metastatic sites before diagnosis. The vast majority of ovarian cancer cases occur in women over 40 and the median age at diagnosis is 63. Additionally, elderly women receive poorer prognoses when diagnosed with ovarian cancer. Despite age being a significant risk factor for the development of this cancer, there are little publi…

    Date Published:
    2018
  • Author(s):
    Karen A. Bailey, Yuliya Klymenko, Peter E. Feist, Amanda B. Hummon, M. Sharon Stack, Zachary D. Schultz
    Abstract:

    Ovarian cancer (OvCa) cells are reported to undergo biochemical changes at the cell surface in response to treatment with lysophosphatidic acid (LPA). Here we use scanning electron microscopy (SEM) and multiplex coherent anti-Stokes Raman scattering (CARS) imaging via supercontinuum excitation to probe morphological changes that result from LPA treatment. SEM images show distinct shedding of microvilli-like features upon treatment with LPA. Analysis of multiplex CARS images can distinguish be…

    Date Published:
    2017
  • Author(s):
    M. Sharon Stack, Jing Yang, William C. Kasberg, Angela Celo, Zhong Liang, Kristal Quispe
    Abstract:

    Membrane type 1 matrix metalloproteinase (MT1-MMP, MMP-14) is a transmembrane collagenase highly expressed in metastatic ovarian cancer and correlates with poor survival. Accumulating evidence shows that the cytoplasmic tail of MT1-MMP is subjected to phosphorylation, and this post-translational modification regulates enzymatic activity at the cell surface. To investigate the potential role of MT1-MMP cytoplasmic residue Thr567 phosphorylation in regulation of metastasis-associated behaviors,…

    Date Published:
    2017