Articles & Publications
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List of files deposited in CurateND that match your search criteria
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- Author(s):
- Jochen H Lorch, Jodi Klessner, J Ken Park, Spiro Getsios, Yvonne L Wu, M Sharon Stack, Kathleen J Green
- Abstract:
The epidermal growth factor receptor (EGFR) has been proposed as a key modulator of cadherin-containing intercellular junctions, particularly in tumors that overexpress this tyrosine kinase. Here the EGFR tyrosine kinase inhibitor PKI166 and EGFR blocking antibody C225, both of which are used clinically to treat head and neck cancers, were used to determine the effects of EGFR inhibition on intercellular junction assembly and adhesion in oral squamous cell carcinoma cells. EGFR inhibition res…
- Date Published:
- 2004-08-27
- Record Visibility:
- Public
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- Author(s):
- S Ghosh, R Brown, J C Jones, S M Ellerbroek, M S Stack
- Abstract:
Expression of urinary-type plasminogen activator (uPA) and its receptor (uPAR) is correlated with matrix proteolysis, cell adhesion, motility, and invasion. To evaluate the functional link between adhesion and proteolysis in gingival keratinocytes (pp126), cells were treated with immobilized integrin antibodies to induce integrin clustering. Clustering of alpha(3) and beta(1) integrin subunits, but not alpha(2), alpha(5), alpha(6), or beta(4), enhanced uPA secretion. Bead-immobilized laminin-…
- Date Published:
- 2000-02-02
- Record Visibility:
- Public
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- Author(s):
- T L Moser, J J Enghild, S V Pizzo, M S Stack
- Abstract:
This study describes the binding of plasminogen and tissue-type plasminogen activator (t-PA) to the extracellular matrix proteins fibronectin and laminin. Plasminogen bound specifically and saturably to both fibronectin and laminin immobilized on microtiter wells, with Kd(app) values of 115 and 18 nM, respectively. Limited proteolysis by endoproteinase V8 coupled with ligand blotting analysis showed that both plasminogen and t-PA preferentially bind to a 55-kDa fibronectin fragment and a 38-k…
- Date Published:
- 1998-09-05
- Record Visibility:
- Public
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4
Article
- Author(s):
- S M Ellerbroek, Y I Wu, C M Overall, M S Stack
- Abstract:
Type I collagen stimulation of pro-matrix metalloproteinase (pro-MMP)-2 activation by ovarian cancer cells involves beta(1) integrin receptor clustering; however, the specific cellular and biochemical events that accompany MMP processing are not well characterized. Collagenolysis is not required for stimulation of pro-MMP-2 activation, and denatured collagen does not elicit an MMP-2 activation response. Similarly, DOV13 cells bind to intact collagen utilizing both alpha(2)beta(1) and alpha(3)…
- Date Published:
- 2001-07-06
- Record Visibility:
- Public
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- Author(s):
- Subhendu Mukhopadgyay, Hidayatullah G Munshi, Suman Kambhampati, Antonella Sassano, Leonidas C Platanias, M Sharon Stack
- Abstract:
Matrix metalloproteinases (MMPs) play an important role in the invasive behavior of a number of cancers including oral squamous cell cancer (OSCC), and increased expression of MMP-9 is correlated with invasive and metastatic OSCC. Because calcium is an important regulator of keratinocyte function, the effect of modulating extracellular calcium on MMP-9 expression in OSCC cell lines was evaluated. Increasing extracellular calcium induced a dose-dependent increase in MMP-9 expression in immorta…
- Date Published:
- 2004-08-06
- Record Visibility:
- Public
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- Author(s):
- Timothy N. Young, Salvatore V. Pizzo, M. Sharon Stack
- Abstract:
Several recent investigations have demonstrated that matrix metalloproteinase-2 (MMP-2) binds to the cell surface and undergoes zymogen activation via a plasma membrane-associated activity. The purpose of this study was to determine if association of MMP-2 with the plasma membrane also modulates the catalytic efficiency of the active enzyme. Using density gradient centrifugation, we isolated the plasma membrane fractions of two ovarian adenocarcinoma cell lines, DOV 13 and OVCA 432, previousl…
- Date Published:
- 01-20-1995
- Record Visibility:
- Public
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7
Article
- Author(s):
- M S Stack, R D Gray
- Abstract:
A fluorogenic substrate for vertebrate collagenase and gelatinase, Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2, was designed using structure-activity data obtained from studies with synthetic inhibitors and other peptide substrates of collagenase. Tryptophan fluorescence was efficiently quenched by the NH2-terminal dinitrophenyl group, presumably through resonance energy transfer. Increased fluorescence accompanied hydrolysis of the peptide by collagenase or gelatinase purified from culture medium …
- Date Published:
- 1989-03-15
- Record Visibility:
- Public
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8
Article
- Author(s):
- M.S. Stack, D.A. Johnson
- Abstract:
Human lung mast cell tryptase is a trypsin-like serine proteinase that is stored in mast cell granules and released by activated mast cells. Here we report that mast cell tryptase is a potent activator of single-chain urinary-type plasminogen activator (scu-PA, or prourokinase), the zymogen form of urinary-type plasminogen activator (u-PA). Activation was complete within 75 min using an enzyme:substrate molar ratio of 1:50 and was accompanied by cleavage of scu-PA at Lys158-Ile159, generating…
- Date Published:
- 1994-04-01
- Record Visibility:
- Public
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- Author(s):
- Chen Wang, John T Norton, Supurna Ghosh, Julie Kim, Kazuo Fushimi, Jane Y Wu, M Sharon Stack, Sui Huang
- Abstract:
RNA processing is altered during malignant transformation, and expression of the polypyrimidine tract-binding protein (PTB) is often increased in cancer cells. Although some data support that PTB promotes cancer, the functional contribution of PTB to the malignant phenotype remains to be clarified. Here we report that although PTB levels are generally increased in cancer cell lines from multiple origins and in endometrial adenocarcinoma tumors, there appears to be no correlation between PTB l…
- Date Published:
- 2008-07-18
- Record Visibility:
- Public
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- Author(s):
- Ying Dong, Yaowu He, Leonore de Boer, M Sharon Stack, John W Lumley, Judith A Clements, John D Hooper
- Abstract:
Epidermal growth factor (EGF) activation of the EGF receptor (EGFR) is an important mediator of cell migration, and aberrant signaling via this system promotes a number of malignancies including ovarian cancer. We have identified the cell surface glycoprotein CDCP1 as a key regulator of EGF/EGFR-induced cell migration. We show that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role…
- Date Published:
- 2012-03-23
- Record Visibility:
- Public
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- Author(s):
- Maria V Barbolina, Yiuying Liu, Hilal Gurler, Mijung Kim, Andre A Kajdacsy-Balla, Lisa Rooper, Jaclyn Shepard, Michael Weiss, Lonnie D Shea, Peter Penzes, Matthew J Ravosa, M Sharon Stack
- Abstract:
Cells respond to changes in the physical properties of the extracellular matrix with altered behavior and gene expression, highlighting the important role of the microenvironment in the regulation of cell function. In the current study, culture of epithelial ovarian cancer cells on three-dimensional collagen I gels led to a dramatic down-regulation of the Wnt signaling inhibitor dickkopf-1 with a concomitant increase in nuclear β-catenin and enhanced β-catenin/Tcf/Lef transcriptional activity…
- Date Published:
- 2013-01-04
- Record Visibility:
- Public
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12
Article
- Author(s):
- Hidayatullah G Munshi, Yi I Wu, Edgardo V Ariztia, M Sharon Stack
- Abstract:
Activation of matrix metalloproteinase 2 (MMP-2) has been shown to play a significant role in the behavior of cancer cells, affecting both migration and invasion. The activation process requires multimolecular complex formation involving pro-MMP-2, membrane type 1-MMP (MT1-MMP), and tissue inhibitor of metalloproteinases-2 (TIMP-2). Because calcium is an important regulator of keratinocyte function, we evaluated the effect of calcium on MMP regulation in an oral squamous cell carcinoma line (…
- Date Published:
- 2002-11-01
- Record Visibility:
- Public