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  • Author(s):
    Michael Hildreth
    Abstract:

    This report reflects the deliberations and conclusions of an NSF-wide Workshop to explore the prospects for a common response to the requirements for public access to research data. Representatives of almost all of the NSF Directorates convened in Alexandria, VA, February 22 and 23, 2018, to review a diverse and multi-disciplinary collection of projects and workshops that have been conducted in the recent past. All of these activities were focused on aspects of public access to research data,…

    Date Created:
    2019-02-18
    Resource Type
    Report
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large imaging depth, but also the strengths of FLIM including the ability to discriminate different fluorophores with similar emission spectra. In FLIM, the frequency-domain (FD) method, which relies on periodic intensity-modulated excitation light and i…

    Date Created:
    2015-10-22
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, conventional fluorescence microscopes cannot satisfy these needs due to physical limits on resolution, speed, information, and depth. In this work, we aim to overcome these limits using novel super-resolution microscopy and high-speed quantitative…

    Date Created:
    2018-04-09
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Multiphoton microscopy (MPM) is a widely used in vivo imaging technique in biological and medical applications. In the case of two-photon excitation fluorescence, two lower-energy photons excite a fluorophore which in turn emits a single higher-energy photon, whose rate depends quadratically on the excitation intensity, thus enabling higher resolution with narrower point spread function (PSF) than conventional one-photon fluorescence microscopy. Fluorescence lifetime imaging microscopy (FLIM)…

    Date Created:
    2016-04-04
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform with super-sensitivity and super-resolution capabilities to quantitatively obtain this information at the intracellular level. First, we present a simple MPM oxygen imaging probe compatible with aqueous biological media based on water-soluble rut…

    Date Created:
    2017-03-30
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang
    Abstract:

    Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high cost. In this research, we propose and demonstrate a new kind of super-resolution fluorescence microscopy that can be easily implemented and requires neither additional hardware nor complex post-processing. The microscopy is based on the principle o…

    Date Created:
    2017-10-19
    Resource Type
    Presentation
  • Author(s):
    Yide Zhang, Scott S. Howard, Cody J. Smith
    Abstract:

    Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment. Other than a traditional confocal or two-photon microscope, this approac…

    Date Created:
    2019-01-09
    Resource Type
    Software
  • Abstract:

    PNDP 1300: Program for the August 2, 1845 Graduation Ceremonies at the University of Notre Dame, Notre Dame, Indiana, United States of America

    Date Published:
    1845-08-02
    Resource Type
    Document
  • Abstract:

    PNDP 1300: Program for the June 10, 1918 Graduation Ceremonies at the University of Notre Dame, Notre Dame, Indiana, United States of America

    Date Published:
    1918-06-10
    Resource Type
    Document
  • Abstract:

    PNDP 1300: Program for the June 11, 1917 Graduation Ceremonies at the University of Notre Dame, Notre Dame, Indiana, United States of America

    Date Published:
    1917-06-11
    Resource Type
    Document
  • Abstract:

    PNDP 1300: Program for the June 14, 1915 Graduation Ceremonies at the University of Notre Dame, Notre Dame, Indiana, United States of America

    Date Published:
    1915-06-14
    Resource Type
    Document
  • Abstract:

    PNDP 1300: Program for the July 2, 1853 Graduation Ceremonies at the University of Notre Dame, Notre Dame, Indiana, United States of America

    Date Published:
    1853-07-02
    Resource Type
    Document