High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac

Patent

Description

The present invention provides molecular methods for efficiently transforming the genome of common disease-transmitting parasites, such as Plasmodium falciparum. The transformation efficiencies are improved up to 100 times over those conventionally known. The methods provide high saturation of the target parasite genome, of 50% or greater, and target non-specifically TTAA-rich sites in the parasite genome. The invention also discloses a model that may be used to functionally annotate the genome of the Plasmodium falciparum, thus permitting the design and screening of compounds that may be useful in the control and inhibiting of diseases caused and transmitted by these parasites, including malaria. Highly efficient and multi-site integrating transposons, particularly piggyBac transposons, which provide for random and multi-site integration into parasite genomes in the presence of a helper plasmid, are also presented.

Attributes

Attribute NameValues
Title
  • High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac

Patent Number
  • US 7932088 B2

USPTO Link
Inventor
  • John H. Adams

  • Malcolm J. Fraser

  • Bharath Balu

  • Douglas A. Shoue

Inventor From Local Institution
  • Malcolm J. Fraser

Other Application
  • 11/410,333

Claims
  • 29

Classification (US Patent)
  • 435/473; 800/291;

Cooperative Patent Classification codes
  • A01K 67/0335 (20130101); C12N 9/22 (20130101); A01K 2217/054 (20130101); C12N 2800/60 (20130101); A01K 2267/03 (20130101); A01K 2227/70 (20130101)

International Patent Classification codes
  • C12N 15/74 (20060101); C12N 15/82 (20060101); C12N 15/87 (20060101); A01H 1/00 (20060101)

Language
  • eng

Date Issued
  • 2011-04-26

Publisher
  • United States Patent and Trademark Office

Assignee
  • University of Notre Dame Du Lac

Access Rights Open Access
Content License
Departments and Units

Files

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