US7932088.pdf (1.95 MB)
High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac
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posted on 2016-06-07, 00:00 authored by Bharath Balu, Douglas A. Shoue, John H. Adams, Malcolm J. FraserThe present invention provides molecular methods for efficiently transforming the genome of common disease-transmitting parasites, such as Plasmodium falciparum. The transformation efficiencies are improved up to 100 times over those conventionally known. The methods provide high saturation of the target parasite genome, of 50% or greater, and target non-specifically TTAA-rich sites in the parasite genome. The invention also discloses a model that may be used to functionally annotate the genome of the Plasmodium falciparum, thus permitting the design and screening of compounds that may be useful in the control and inhibiting of diseases caused and transmitted by these parasites, including malaria. Highly efficient and multi-site integrating transposons, particularly piggyBac transposons, which provide for random and multi-site integration into parasite genomes in the presence of a helper plasmid, are also presented.
History
Patent Number
US 7932088 B2Other Application
11/410,333Inventor
John H. Adams Malcolm J. Fraser Bharath Balu Douglas A. ShoueInventor from Local Institution
Malcolm J. FraserAssignee
University of Notre Dame Du LacDate Modified
2016-06-07Language
- English
Claims
29Publisher
United States Patent and Trademark OfficeCooperative Patent Classification Codes
A01K 67/0335 (20130101); C12N 9/22 (20130101); A01K 2217/054 (20130101); C12N 2800/60 (20130101); A01K 2267/03 (20130101); A01K 2227/70 (20130101)Contributor
Malcolm J. FraserInternational Patent Classification Codes
C12N 15/74 (20060101); C12N 15/82 (20060101); C12N 15/87 (20060101); A01H 1/00 (20060101)US Patent Classification Codes
435/473; 800/291;Usage metrics
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