Shear Stress in Bone Marrow has a Dose Dependent Effect on cFos Gene Expression in In Situ Culture

Article

Abstract

Introduction: Mechanical stimulation of bone is necessary to maintain its mass and architecture. Osteocytes within the mineralized matrix are sensors of mechanical deformation of the hard tissue, and communicate with cells in the marrow to regulate bone remodeling. However, marrow cells are also subjected to mechanical stress during whole bone loading, and may contribute to mechanically regulated bone physiology. Previous results from our laboratory suggest that mechanotransduction in marrow cells is sufficient to cause bone formation in the absence of osteocyte signaling. In this study, we investigated whether bone formation and altered marrow cell gene expression response to stimulation was dependent on the shear stress imparted on the marrow by our loading regime. Methods: Porcine trabecular bone explants were cultured in an in situ bioreactor for 5 or 28 days with stimulation twice daily. Gene expression and bone formation were quantified and compared to unstimulated controls. Correlation was used to assess the dependence on shear stress imparted by the loading regime calculated using computational fluid dynamics models. Results: Vibratory stimulation resulted in a higher trabecular bone formation rate (p = 0.01) and a greater increase in bone volume fraction (p = 0.02) in comparison to control explants. Marrow cell expression of cFos increased with the calculated marrow shear stress in a dose-dependent manner (p = 0.002). Conclusions: The results suggest that the shear stress due to interactions between marrow cells induces a mechanobiological response. Identification of marrow cell mechanotransduction pathways is essential to understand healthy and pathological bone adaptation and remodeling.

Attributes

Attribute NameValues
Creator
  • Kimberly Curtis

  • Thomas Coughlin

  • Mary Varsanik

  • Glen Niebur

Journal or Work Title
  • Cellular and Molecular Bioengineering

Volume
  • 12

Issue
  • 6

First Page
  • 559

Last Page
  • 568

Number of Pages
  • 9

ISSN
  • 18655033

Publication Date
  • 2019-08

Subject
  • Magellan SEM

Publisher
  • Biomedical Engineering Society

Date Created
  • 2020-04-08

Language
  • English

Departments and Units
Record Visibility Public
Content License
  • All rights reserved

Digital Object Identifier

doi:10.7274/r0-bgdn-h297

This DOI is the best way to cite this article.