Rapid detection of viable bacteria system and method

Patent

Description

An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample.

Attributes

Attribute NameValues
Title
  • Rapid detection of viable bacteria system and method

Patent Number
  • US 8635028 B2

USPTO Link
Inventor
  • Shramik Sengupta

  • Hsueh-Chia Chang

Inventor From Local Institution
  • Hsueh-Chia Chang

Other Application
  • 12/896,188

Prior Publication Date
  • 2011-04-07

Prior Publication Number
  • US 20110081676 A1

Claims
  • 18

Classification (US Patent)
  • 702/19

Cooperative Patent Classification codes
  • C12Q 1/04 (20130101); G01N 33/5438 (20130101); C12Q 1/06 (20130101); B01L 3/502715 (20130101)

International Patent Classification codes
  • G01N 33/50 (20060101)

Language
  • English

Date Issued
  • 2014-01-21

Publisher
  • U.S. Patent and Trademark Office

Assignee
  • The Curators of the University of Missouri (Columbia, MO)

  • University of Notre Dame du Lac

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