Influence of Extracellular Polymeric Substances on the Adsorption of Cadmium onto Three Bacterial Species

Article

Abstract

In this study, we measured the effect of EPS on Cd and proton adsorption behaviors by measuring the extent of adsorption onto biomass with and without the EPS removed via a cation exchange resin. We conducted both Cd adsorption experiments and potentiometric titrations of biomass using three common bacterial species: one Gram-positive (Bacillus subtilis) and two Gram-negative (Shewanella oneidensis, Pseudomonas putida) species. The Cd adsorption experiments were con- ducted as a function of metal loading in order to probe whether environmentally-low metal load- ings lead to different adsorption mechanisms and roles for EPS than the higher metal loadings of most previous adsorption studies. We suspended each biomass sample in a solution of dissolved Cd in 0.01 M NaClO4 at metal loadings of 1, 2, 5, and 74 lmol/g. Surface complexation modeling (SCM) was used to determine stability constants for the important Cd-bacteria complexes, and the effect of metal loading on the resulting calculated stability constant values was determined. In general, the measured bulk Cd adsorption behavior is unaffected by EPS removal. However, our potentiometric titration results suggest that EPS removal does alter the distribution of site types, but not the mass-normalized total site concentration within the biomass. SCM suggests that high affinity sulfhydryl sites control Cd binding under low metal loading conditions for B. subtilis and P. putida, and that sulfhydryl sites are present both on the cells and within the EPS for these species. Conversely, the SCM results suggest that Cd-sulfhydryl binding is un-important on the EPS of S. oneidensis.

Attributes

Attribute NameValues
Creator
  • Margaret Butzen

  • Jeremy Fein

Journal or Work Title
  • Geomicrobiology Journal

Volume
  • 36

Issue
  • 5

First Page
  • 412

Last Page
  • 422

ISSN
  • 15210529

Publication Date
  • 2019-01

Subject
  • SEM

Date Created
  • 2019-08-23

Language
  • English

Departments and Units
Record Visibility and Access Public
Content License
  • All rights reserved

Digital Object Identifier

doi:10.1080/01490451.2018.1564804

This DOI is the best way to cite this article.