Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors

Article

Abstract

Phosphatidylserine externalization is an early molecular signature for apoptosis. In many retinal degenerative diseases, photoreceptor neurons die by apoptosis. Here, we report utility of the phosphatidylserine-binding conjugate of Bis(zinc(II)-dipicolylamine (Zn-DPA) with Texas-red (PSVue-550) in transiently labeling apoptotic photoreceptors in living pigmented or albino rats and mice with retinal degeneration. Applying PSVue-550 as eyedrop is non-toxic and eliminates need for intraocular injection. PSVue-550 fluorescence specifically and transiently labeling dying retinal photoreceptors is detectable in anesthetized animals using standard retinal or whole small animal imaging systems. Importantly, prior PSVue-550 eyedrop administration and imaging does not affect repeat testing. Altogether, our results establish PSVue-550 imaging as a completely non-invasive method that provides the opportunity to longitudinally monitor retinal photoreceptor cell death in preclinical studies.

Attributes

Attribute NameValues
Creator
  • Francesca Manzoni

  • Claudia Muller

  • Jonathan DeAssis

  • Deborah Lew

  • W. Matthew Leevy

  • Silvia Finnegan

Journal or Work Title
  • Scientific Reports

Volume
  • 9

Issue
  • 1

First Page
  • 1

Last Page
  • 9

Number of Pages
  • 8

ISSN
  • 20452322

ISBN
  • 4159801838

Publication Date
  • 2019-02

Subject
  • In Vivo Xtreme

Publisher
  • Nature

Date Created
  • 2019-12-16

Language
  • English

Departments and Units
Record Visibility and Access Public
Content License
  • All rights reserved

Digital Object Identifier

doi:10.1038/s41598-018-38363-z

This DOI is the best way to cite this article.