Enhancement of Genome Investigations and Analysis of Diapause Induction in Culex pipiens Mosquitoes

Doctoral Dissertation


Mosquitoes in the Culex pipiens complex are major vectors for a number of important human pathogens. Due to the paucity of vaccines against many mosquito-borne diseases, control of vector populations remains an important means for disease prevention. Members of this species complex are among the most geographically widespread mosquitoes, worldwide. There are two major species in the Cx. pipiens complex: Cx. quinquefasciatus (Say), the southern house mosquito, which occupies tropical to warm temperate regions; and Cx. pipiens (L), the northern house mosquito, which occupies temperate regions. Cx. pipiens females, in response to environmental cues, enter diapause to endure the adverse conditions associated with winter in temperate regions. The rationale for this research is the elucidation of the genetic mechanisms driving diapause induction could provide novel targets for genetic control measures as well as provide additional insights into the forces influencing their geographic distribution.

My first aim was to develop tools to enhance genomic studies in Cx. pipiens complex mosquitoes. We used data generated by the Cx. quinquefasciatus (Say) genome project for in silico development of over 75 microsatellite markers. Cross-species utility of these markers was tested in populations from Trinidad and the USA. In addition, we used 6 F1 intercross mapping populations to map the location of these microsatellites, which subsequently allowed us to assign 10.4% of the genome assembly and 13.5% of the protein coding genes to chromosome position. We constructed a BAC library (NDJ) with approximately 10.1 fold coverage of the Cx. quinquefasciatus genome. We used 29 microsatellites and five gene markers to help estimate genome coverage.

My second aim was to identify candidate mechanisms driving diapause induction in Cx. pipiens mosquitoes. We used custom microarray probe sets representing the Cx. quinquefasciatus transcriptome to measure gene expression following exposure to standard and diapause-inducing conditions during the early pupal stage, a photosensitive stage for diapause induction. We identified 1,130 genes that were differentially expressed, and we used the expression of these genes across three time points to construct a gene co-expression network comprising five modules. GO term and pathway enrichment analysis of the network modules revealed potential mechanisms driving diapause induction in Cx. pipiens mosquitoes, including TGF-β signaling and the chromatin modification complexes SIN3 and NuRD.


Attribute NameValues
  • etd-06022014-110751

Author Paul V Hickner
Advisor David W. Severson
Contributor John G. Duman , Committee Member
Contributor Molly Duman Scheel, Committee Member
Contributor Jeanne Romero-Severson, Committee Member
Contributor David W. Severson, Committee Chair
Contributor Michael Pfrender , Committee Member
Degree Level Doctoral Dissertation
Degree Discipline Biological Sciences
Degree Name PhD
Defense Date
  • 2014-05-13

Submission Date 2014-06-02
  • United States of America

  • microsatellites

  • Culex quinquefasciatus

  • linkage map

  • BAC library

  • overwintering

  • University of Notre Dame

  • English

Record Visibility Public
Content License
  • All rights reserved

Departments and Units


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