Genetic Architecture and Transcriptomic Basis of Cadmium Response in Daphnia magna

Doctoral Dissertation

Abstract

Natural Populations are increasingly challenged by rapidly changing environmental conditions. Toxic compounds produced from industrial and urban activities present a significant challenge to aquatic organisms. Heavy metals, in particular, are common stressors that play a major role in environmental damage. Since aquatic systems are the major sinks of industrial effluents they are often more highly impacted by heavy metals than terrestrial ecosystems. Understanding the fitness consequences of heavy metal exposure requires knowledge of the physiological responses of organisms and the genetic basis of these responses. This information is critical to environmental scientists interested in predicting the long-term consequences of altered environments, and to regulatory agencies constructing predictive models to assess the impact of pollutants on human and environmental health.

We evaluate the underlying genetic architecture and transcriptional responses of the freshwater microcrustacean Daphnia magna to cadmium exposure in order to identify genes and pathways involved in the physiological response to this common environmental contaminant. We use QTL-mapping in recombinant lines derived from parental lines that differ in their response to cadmium exposure to detect genome regions associated with survivorship, and cadmium assimilation and depuration rates. In addition, we use a functional genomic approach with RNA-Seq data to examine transcriptional responses to cadmium exposure. Our investigation of organismal response to cadmium will enhance our understanding of the effects of stressful environments on a widely distributed and ecologically relevant aquatic organism, and will add valuable information to current ecotoxicology research.

Attributes

Attribute NameValues
Alternate Title
  • Genetic and transcriptomic basis of cadmium response in Daphnia magna

Author Sayanty Roy
Contributor David Severson, Committee Member
Contributor Michael Pfrender, Research Director
Contributor Michael Ferdig, Committee Member
Contributor John Duman, Committee Member
Degree Level Doctoral Dissertation
Degree Discipline Biological Sciences
Degree Name Doctor of Philosophy
Defense Date
  • 2016-04-07

Submission Date 2016-04-14
Record Visibility and Access Public
Content License
  • All rights reserved

Departments and Units

Files

Please Note: You may encounter a delay before a download begins. Large or infrequently accessed files can take several minutes to retrieve from our archival storage system.