Release of Applied Mechanical Loading Stimulates Intercellular Calcium Waves in Drosophila Wing Discs

Article

Abstract

Mechanical forces are critical but poorly understood inputs for organogenesis and wound healing. Calcium ions (Ca2+) are critical second messengers in cells for integrating environmental and mechanical cues, but the regulation of Ca2+ signaling is poorly understood in developing epithelial tissues. Here we report a chip-based regulated environment for microorgans that enables systematic investigations of the crosstalk between an organ’s mechanical stress environment and biochemical signaling under genetic and chemical perturbations. This method enabled us to define the essential conditions for generating organ-scale intercellular Ca2+ waves in Drosophila wing discs that are also observed in vivo during organ development. We discovered that mechanically induced intercellular Ca2+ waves require fly extract growth serum as a chemical stimulus. Using the chip-based regulated environment for microorgans, we demonstrate that not the initial application but instead the release of mechanical loading is sufficient, but not necessary, to initiate intercellular Ca2+ waves. The Ca2+ response depends on the prestress intercellular Ca2+ activity and not on the magnitude or duration of the mechanical stimulation applied. Mechanically induced intercellular Ca2+ waves rely on IP3R-mediated Ca2+-induced Ca2+ release and propagation through gap junctions. Thus, intercellular Ca2+ waves in developing epithelia may be a consequence of stress dissipation during organ growth.

Attributes

Attribute NameValues
Creator
  • Cody E. Narcisco

  • Nicholas M. Contento

  • Thomas J. Storey

  • David J. Hoelzle

  • Jeremiah J. Zartman

Journal or Work Title
  • Biophysical Journal

Volume
  • 113

Issue
  • 2

First Page
  • 491

Last Page
  • 501

Publication Date
  • 2017-07

Publisher
  • Biophysical Society

Date Created
  • 2018-10-29

Language
  • English

Departments and Units
Record Visibility and Access Public
Content License
  • All rights reserved

Digital Object Identifier

doi:10.1016/j.bpj.2017.05.051

This DOI is the best way to cite this article.