Yarrowia lipolytica as a Microbial Platform for Waste-to-Value Biotransformation

Master's Thesis


Yarrowia lipolytica is an oleaginous, nonconventional yeast with impressive lipid biosynthesis, extremophilic characteristics and the ability to consume a wide range of substrates. These characteristics make Y. lipolytica an effective microbial platform for waste-to-value biotransformation of waste biomass and food waste-derived volatile fatty acids (VFAs). Ionic liquid (IL) carryover from lignocellulosic biomass pretreatment inhibits microbial biotransformation of waste biomass into valuable fuels and chemicals during simultaneous saccharification and fermentation. To overcome this challenge, we used evolutionary engineering to develop an IL tolerant strain of Y. lipolytica that can grow at a competitive growth rate in the presence of ≤14% IL (v/v). We also investigated the potential of Y. lipolytica as an oleaginous cell platform for bioconversion of food waste-derived VFAs, produced during anerobic digestion, into biolipids for biodiesel. We found that Y. lipolytica can utilize sole VFAs as a cost-effective substrate producing lipid contents up to 25% of cell biomass.


Attribute NameValues
Author Brooke Stemple
Contributor Na Wei, Research Director
Degree Level Master's Thesis
Degree Discipline Civil and Environmental Engineering and Earth Sciences
Degree Name Master of Science in Environmental Engineering
Banner Code

Defense Date
  • 2018-12-14

Submission Date 2019-03-11
  • Volatile fatty acids (VFAs)

  • Ionic liquid simultaneous saccharification and fermentation (IL-SSF)

  • Yarrowia lipolytica

Record Visibility Public
Content License
  • All rights reserved

Departments and Units
Catalog Record


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