Systems and methods for bead-free detection of nucleotides



Methods and systems of quantifying a target material in solution include detection of a size change of a hybridized nucleic acid complex, without the use of nanobeads. In particular, the examples include providing a plurality of nucleic acid fragments and a species-specific oligonucleotide tags, measuring the size of the nucleic acid fragments and/or oligonucleotides to predetermine a standard distribution of the solution(s), introducing the oligonucleotides in a solution containing nucleic acid target materials and/or non-target materials, and hybridizing the oligonucleotides with the species-specific target material if present in the solution. The size of the nucleic acid complexes in solution are then measured after hybridization, and the presence or non-presence of the species-specific target material is detected and/or quantified by comparing the measured size of the nucleic acid complexes after hybridization to the standard distribution.


Attribute NameValues
  • Systems and methods for bead-free detection of nucleotides

Patent Number
  • US 9447456 B2

  • Carol Tanner

  • Steven Ruggiero

Inventor From Local Institution
  • Carol Tanner

  • Steven Ruggiero

Other Application
  • 14/114,174

Prior Publication Date
  • 2014-05-01

Prior Publication Number
  • US 20140120539 A1

  • 16

Cooperative Patent Classification codes
  • G01N 21/59 (20130101); C12Q 1/6851 (20130101); C12Q 1/6816 (20130101); C12Q 1/6816 (20130101); C12Q 2563/155 (20130101); G01N 15/0205 (20130101); G01N 2015/0038 (20130101); C12Q 2537/165 (20130101); C12Q 2545/114 (20130101); C12Q 2565/601 (20130101)

International Patent Classification codes
  • C12Q 1/68 (20060101); C07H 21/00 (20060101); G01N 33/48 (20060101)

  • English

Date Issued
  • 2016-09-20

  • U.S. Patent and Trademark Office

  • University of Notre Dame du Lac

Record Visibility Public
Content License
  • All rights reserved

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