9,447,456 OCR.pdf (1.57 MB)
Systems and methods for bead-free detection of nucleotides
standard
posted on 2017-08-01, 00:00 authored by Carol Tanner, Steven RuggieroMethods and systems of quantifying a target material in solution include detection of a size change of a hybridized nucleic acid complex, without the use of nanobeads. In particular, the examples include providing a plurality of nucleic acid fragments and a species-specific oligonucleotide tags, measuring the size of the nucleic acid fragments and/or oligonucleotides to predetermine a standard distribution of the solution(s), introducing the oligonucleotides in a solution containing nucleic acid target materials and/or non-target materials, and hybridizing the oligonucleotides with the species-specific target material if present in the solution. The size of the nucleic acid complexes in solution are then measured after hybridization, and the presence or non-presence of the species-specific target material is detected and/or quantified by comparing the measured size of the nucleic acid complexes after hybridization to the standard distribution.
History
Patent Number
US 9447456 B2Other Application
14/114,174Inventor
Carol Tanner Steven RuggieroInventor from Local Institution
Carol Tanner Steven RuggieroAssignee
University of Notre Dame du LacDate Modified
2017-08-01Language
- English
Claims
16Prior Publication Number
US 20140120539 A1Publisher
U.S. Patent and Trademark OfficeCooperative Patent Classification Codes
G01N 21/59 (20130101); C12Q 1/6851 (20130101); C12Q 1/6816 (20130101); C12Q 1/6816 (20130101); C12Q 2563/155 (20130101); G01N 15/0205 (20130101); G01N 2015/0038 (20130101); C12Q 2537/165 (20130101); C12Q 2545/114 (20130101); C12Q 2565/601 (20130101)Contributor
Carol Tanner|Steven RuggieroInternational Patent Classification Codes
C12Q 1/68 (20060101); C07H 21/00 (20060101); G01N 33/48 (20060101)Usage metrics
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