Adsorbing, Desorbing, Jamming, and Burning DNA Origami

A single computer chip has more components than there are people in the world. Available space on the silicon chip is reaching the limit for adding new features. Scientists and engineers continue to look towards understanding single molecules, nanostructures, thin films, and microstructures as components with which to build smaller, more robust circuits. In this regard, DNA origami has taken shape as a promising star. The nanostructure, made entirely from polynucleotides, forms by self-assembly, yields billions of copies in a small drop, and can be functionalized, all in a one pot synthesis. The placement of new and smaller features on a silicon chip is possible with directed DNA origami binding onto silicon surfaces. Lithographically patterned anchor pads[1] and complimentary shapes[2] have been shown to direct the placement of individual origami on modified silicon surfaces. However, binding errors and non-uniform orientation still occur. Therefore, it is important to understand and to develop methods to control DNA origami binding. I will present the first set of results on the characteristics of DNA origami adsorption to and desorption from silicon and mica substrates, which speaks to the stability of these structures on surfaces, and their ability to anneal during deposition. I will also compare the maximum surface coverage of DNA origami with predictions based on a random sequential adsorption model. In addition, I worked on burn-in doping of phosphorus and electrical manipulation of DNA origami, and I will present this data.

Finally, I include a time capsule which places the reader in 1896 Chicago, and gives insight on the University of Chicago's progressive influence on science and STEM education in Chicago and the city of Chicago's political support at that time. Borrowing from those changes in attitudes about science education, I propose that nanotechnology curriculum development could help turn around low achievement scores in science and math among African American students in the city. I discuss an engaging tactile activity modeled after the Atomic Force Microscope that I developed as a University of Notre Dame-NSF GK-12 Graduate Fellow. Details of how to make and use this teaching tool are provided.