High Sensitivity CZE-ESI-MS/MS: Method Development and Applications

Master's Thesis

Abstract

Proteomic studies are usually performed using LC-MS, UPLC-MS through out the past decades. However, electrophoresis has emerged as a high-resolution separation method that can be coupled to various detection method for a thorough analysis of the proteome of any biological system. The Dovichi group uses capillary zone electrophoresis (CZE) coupled to a mass spectrometry through electrospray ionization (ESI) to perform bottom-up analyses of complex proteomes.

In this work, we demonstrate the reproducible detection of two fragment ions from injection of 1 zmol (600 ions) of angiotensin onto a CZE system coupled to a Q-Exactive HF using our electrokinetically-pumped nanoelectrospray interface. The methods described herein establish the state of the art detection limits of capillary electrophoresis with mass spectrometry.

Attributes

Attribute NameValues
Author Emily Amenson-Lamar
Contributor Norman J. Dovichi, Research Director
Degree Level Master's Thesis
Degree Discipline Chemistry and Biochemistry
Degree Name Master of Science
Banner Code
  • MS-CHEM

Defense Date
  • 2021-04-16

Submission Date 2021-04-20
Subject
  • Analytical Chemistry

  • limits of detection

  • CZE-ESI-MS/MS

Language
  • English

Record Visibility Public
Content License
  • All rights reserved

Departments and Units
Catalog Record

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