University of Notre Dame
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on University of Notre Dame and we can't guarantee its availability, quality, security or accept any liability.

Saturation-compensated measurements for fluorescence lifetime imaging microscopy

journal contribution
posted on 2018-10-31, 00:00 authored by Aamir A. Khan, David Benirschke, Genevieve D. Vigil, Lina Cao, Patrick Fay, Scott S. Howard, Tahsin Ahmed
Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM at high excitation powers. In this Letter, we present an analytical theoretical description of this error and present a method for compensating for this error in order to extract correct lifetime measurements in the limit of fluorophore saturation. We perform a series of simulations and experiments to validate our methods. The simulations and experiments show a 13.2× and a 2.6× increase in excitation range, respectively. The presented method is based on algorithms that can be easily applied to existing FLIM setups.

History

Date Modified

2018-12-07

Language

  • English

Publisher

OSA: The Optical Society

Additional Groups

  • Integrated Imaging Facility

Usage metrics

    Electrical Engineering

    Categories

    No categories selected

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC