Antibody purification via affinity chromatography

Patent

Description

Embodiments herein provide methods of purifying monoclonal and polyclonal antibodies (e.g., immunoglobulins) from biological fluids, such as cell lysates, cell supernatant and ascites fluids, using small molecule affinity chromatography. Various embodiments disclose a class of small molecules that selectively bind a nucleotide binding site that is inherent to all immunoglobulins, and in various embodiments, methods are disclosed that use one of these small molecules as a capture molecule in small molecule affinity chromatography. In some embodiments, the small molecule may be an indole, and in particular embodiments, the small molecule may be indole-3-butyric acid.

Attributes

Attribute NameValues
Title
  • Antibody purification via affinity chromatography

Patent Number
  • US 9598460 B2

USPTO Link
Inventor
  • Z. Basar Bilgicer

  • Tanyel Kiziltepe Bilgicer

  • Nathan J. Alves

  • Jonathan D. Ashley

  • Michael W. Handlogten

Inventor From Local Institution
  • Z. Basar Bilgicer

  • Tanyel Kiziltepe Bilgicer

  • Nathan J. Alves

  • Jonathan D. Ashley

  • Michael W. Handlogten

Other Application
  • 13/979,919

Prior Publication Date
  • 2013-11-21

Prior Publication Number
  • US 20130309699 A1

Claims
  • 18

Cooperative Patent Classification codes
  • C07K 1/22 (20130101); A61K 39/39591 (20130101); C07K 16/065 (20130101); C07K 16/2887 (20130101); C07K 16/32 (20130101); C07K 1/14 (20130101)

International Patent Classification codes
  • C07K 1/14 (20060101); A61K 39/395 (20060101); C07K 16/06 (20060101); C07K 1/22 (20060101); C07K 16/28 (20060101); C07K 16/32 (20060101

Language
  • English

Date Issued
  • 2017-03-21

Publisher
  • U.S. Patent and Trademark Office

Assignee
  • University of Notre Dame du Lac

Record Visibility and Access Public
Content License
  • All rights reserved

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