Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac

Patent

Description

The present invention provides a method for transforming an insect genome that has a much enhanced transformation frequency. The vectors and plasmids employed in the method are further described as transposition vectors that include a minimal amount of nucleotide sequence homologous to a 5’ region and a 3’ region of a native piggyBac nucleic acid sequence. The transformed cells or embryos may also be developed into transgenic organisms. Disclosed are minimal piggyBac-based plasmid constructs that comprises a minimal nucleic acid sequence homologous to a 5’ end of a piggyBac nucleic acid sequence (about 60-80 bp, particularly 66 bp) and a relatively long (300 to about 380 bp, particularly 311 bp or 378 bp) continuous nucleic acid sequence homologous to a 3’ end of a piggyBac native nucleic acid sequence. Methods employing these constructs include the use of a helper plasmid. Transformation frequencies employing the constructs are enhanced 100-fold or higher over that transformation frequency obtained using other than the herein described constructs.

Attributes

Attribute NameValues
Title
  • Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac

Patent Number
  • US 10087463 B2

USPTO Link
Inventor
  • Xu Li

  • Malcolm J. Fraser

Inventor From Local Institution
  • Xu Li

  • Malcolm J. Fraser

Other Application
  • 11/454,947

Prior Publication Date
  • 2010-09-02

Claims
  • 4

Cooperative Patent Classification codes
  • C12N 15/85 (20130101); C12N 15/90 (20130101); C12N 2800/90 (20130101); C12N 2800/204 (20130101)

International Patent Classification codes
  • C12N 15/85 (20060101); C12N 15/90 (20060101)

Language
  • English

Date Issued
  • 2018-10-02

Publisher
  • U.S. Patent and Trademark Office

Assignee
  • University of Notre Dame Du Lac

Record Visibility Public
Content License
  • All rights reserved

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