Elucidation of the selection and roles of synonymous codons in protein biogenesis

Master's Thesis
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Abstract

In this thesis, the importance of rare codons on protein biogenesis as well as cell fitness will be discussed. To test the effects of rare codons, a continuous cell culture device known as a turbidostat was built and optimized within the lab. The turbidostat, as well as batch culture, is used to test the extent to which rare codons affect an antibiotic resistance protein known as chloramphenicol acetyltransferase (CAT). CAT has three known types (I, II, and III), and is responsible for the acetylation of the antibiotic chloramphenicol. There are two main methods that are used to test the fitness effects of different CAT-III constructs, one of which is a competition between two strains, and the other is a CAT assay to directly measure chloramphenicol conversion rates.

Attributes

Attribute NameValues
URN
  • etd-12082014-112058

Author John Herrington
Advisor Patricia L. Clark
Contributor Paul Huber, Committee Member
Contributor Michael Ferdig, Committee Member
Contributor Shahriar Mobashery, Committee Member
Contributor Patricia L. Clark, Committee Member
Degree Level Master's Thesis
Degree Discipline Chemistry and Biochemistry
Degree Name MS
Defense Date
  • 2014-12-01

Submission Date 2014-12-08
Country
  • United States of America

Subject
  • synonymous

  • protein folding

  • chloramphenicol acetyltransferase

  • protein biogenesis

  • continuous cell culture

  • codons

  • turbidostat

Publisher
  • University of Notre Dame

Language
  • English

Record Visibility Public
Content License
  • All rights reserved

Departments and Units

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