ONeilEJ072008D.pdf (4.8 MB)
Synthesis and Study of Membrane Active Compounds
thesis
posted on 2008-07-18, 00:00 authored by Edward J O'NeilThis dissertation describes the design, synthesis, and activity of molecules that interact specifically with vesicle or cellular membranes. The first section describes a series of second generation Zn2+ bis DPA coordination complexes that selectively target negatively charged membrane surfaces including those found in apoptotic cells and bacteria. Several types of conjugates were constructed including fluorescent versions suitable for microscopy, multivalent receptors designed for increased membrane affinity, and lipophilic derivatives. The synthesis and membrane activity of each is discussed. The second portion of this dissertation describes general strategies for the synthesis of sn-2 functionalized phospholipids from DPPC. Phospholipases were utilized for selective headgroup and side chain modification of commercially available phospholipids circumventing a de novo synthetic approach. Several classes of functionalized phosphatidylcholine derivatives were constructed including bolaamphiphiles which in some cases were found to destabilize membranes and chloride anion transporters that function by a novel relay mechanism. Other phospholipid derivatives include phosphatidylserine and phosphatidylethanolamine conjugates that were designed for receptor-mediated delivery of cargo into cells.
History
Date Modified
2017-06-02Defense Date
2008-06-27Research Director(s)
Professor Shahriar MobasheryCommittee Members
Professor Paul Helquist Professor Shahriar Mobashery Professor Richard E. TaylorDegree
- Doctor of Philosophy
Degree Level
- Doctoral Dissertation
Language
- English
Alternate Identifier
etd-07182008-103101Publisher
University of Notre DameProgram Name
- Chemistry and Biochemistry
Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC