No Stressing About Memory: An Investigation of Allopregnanolone and Nitric Oxide Effects on Long-Term Potentiation

Doctoral Dissertation

Abstract

The mechanisms of long-term potentiation were investigated in two sets of experiments. The first determined the effects of the neurosteroid allopregnanolone on long-term potentiation. Results revealed that allopregnanolone inhibits long-term potentiation primarily through its actions on the GABAA receptor. The second experiment used fluorescence microscopy to create a temporal analysis of nitric oxide synthesis in response to high-frequency stimulation and found that the concentration of nitric oxide increased at two separate time periods following the induction of long-term potentiation. These findings explain how it is possible for nitric oxide to be important for increased glutamate release during the initiation of long-term potentiation, as well as gene transcription during later stages. Future research should utilize the methods created in this study to further increase the knowledge of long-term potentiation, as well as determine the mechanisms behind abnormal nitric oxide levels found in various neurological diseases.

Attributes

Attribute NameValues
Author M. Windy McNerney
Advisor Gabriel Radvansky
Contributor Gabriel Radvansky, Research Director
Degree Level Doctoral Dissertation
Degree Discipline Psychology
Degree Name PhD
Defense Date
  • 2012-04-04

Submission Date 2012-04-08
Subject
  • fluorescence microscopy

  • long-term potentiation

  • nitric oxide

  • memory

  • allopregnanolone

Language
  • English

Record Visibility Public
Embargo Release Date
  • 2016-04-18

Content License
  • All rights reserved

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