9856518OCR.pdf (667.78 kB)
Method and apparatus for a nanopipette biosensor
standard
posted on 2018-08-06, 00:00 authored by Hsueh-Chia ChangHsueh-Chia Chang, Satyajyoti Senapati, Shoupeng Liu, Yu Yan, Yunshan WangA nanopipette biosensor capable of detecting a small concentration of target molecules within a sample solution using optical detection methods. The biosensor includes a nanopipette that connects a nanocolloid reservoir containing a nanocolloid solution and a sample reservoir containing a sample solution, where the nanopipette is tapered at the end connected to the sample reservoir. The nanocolloid solution includes nanoparticles functionalized with probes specific to miRNA of the target molecules and reporters. During the detection process, the nanocolloids nanoparticles aggregate such that plasmonic hotspots are formed. These hotspots magnify the reporter signals produced when the probes hybridize with target molecules.
History
Patent Number
US 9856518 B2Other Application
14/283,713Inventor
Hsueh-Chia Chang Shoupeng Liu Satyajyoti Senapati Yunshan Wang Yu YanInventor from Local Institution
Hsueh-Chia Chang Shoupeng Liu Satyajyoti Senapati Yunshan Wang Yu YanAssignee
University of Notre Dame Du LacDate Modified
2018-08-06Language
- English
Claims
20Prior Publication Number
US 20140349287 A1Publisher
U.S. Patent and Trademark OfficeCooperative Patent Classification Codes
C12Q 1/6816 (20130101); C12Q 1/6825 (20130101); C12Q 1/6825 (20130101); C12Q 2525/301 (20130101); C12Q 2563/155 (20130101); C12Q 2565/1015 (20130101); C12Q 2565/601 (20130101)Contributor
Hsueh-Chia Chang|Shoupeng Liu|Satyajyoti Senapati|Yunshan Wang|Yu YanInternational Patent Classification Codes
C12Q 1/68 (20060101)Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC