Method and apparatus for a nanopipette biosensor

Patent

Description

A nanopipette biosensor capable of detecting a small concentration of target molecules within a sample solution using optical detection methods. The biosensor includes a nanopipette that connects a nanocolloid reservoir containing a nanocolloid solution and a sample reservoir containing a sample solution, where the nanopipette is tapered at the end connected to the sample reservoir. The nanocolloid solution includes nanoparticles functionalized with probes specific to miRNA of the target molecules and reporters. During the detection process, the nanocolloids nanoparticles aggregate such that plasmonic hotspots are formed. These hotspots magnify the reporter signals produced when the probes hybridize with target molecules.

Attributes

Attribute NameValues
Title
  • Method and apparatus for a nanopipette biosensor

Patent Number
  • US 9856518 B2

USPTO Link
Inventor
  • Hsueh-Chia Chang

  • Shoupeng Liu

  • Satyajyoti Senapati

  • Yunshan Wang

  • Yu Yan

Inventor From Local Institution
  • Hsueh-Chia Chang

  • Shoupeng Liu

  • Satyajyoti Senapati

  • Yunshan Wang

  • Yu Yan

Other Application
  • 14/283,713

Prior Publication Date
  • 2014-11-27

Prior Publication Number
  • US 20140349287 A1

Claims
  • 20

Cooperative Patent Classification codes
  • C12Q 1/6816 (20130101); C12Q 1/6825 (20130101); C12Q 1/6825 (20130101); C12Q 2525/301 (20130101); C12Q 2563/155 (20130101); C12Q 2565/1015 (20130101); C12Q 2565/601 (20130101)

International Patent Classification codes
  • C12Q 1/68 (20060101)

Language
  • English

Date Issued
  • 2018-01-02

Publisher
  • U.S. Patent and Trademark Office

Assignee
  • University of Notre Dame Du Lac

Record Visibility Public
Content License
  • All rights reserved

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