Rapid Quantitative Protein Detection by Light Transmission Spectroscopy

Article

Abstract

Rapid, sensitive, and quantitative protein detection is critical for many applications in medicine, environmental monitoring, and the food industry. Advancements in detection of proteins include the use of antigen-antibody binding; however, many current methods are time-consuming and have limiting factors such as low sensitivity and the inability to provide absolute values. We present a new high-throughput method for protein detection using light transmission spectroscopy (LTS), which can quantify and size nanoparticles in fluid suspension. LTS can quantify proteins directly and target specific proteins through antigen-antibody binding. This work shows that LTS can distinguish between and quantify bovine serum albumin, its antibody, and the BSA + Ab complex and determine BSA protein concentrations down to 5 μg/mL. We use both Mie and discrete dipole approximation models to provide geometric insight into the binding process.

Attributes

Attribute NameValues
Creator
  • A. E. Deatsch

  • A. J. Shogren

  • S. P. Egan

  • J. L. Tank

  • N. Sun

  • S. T. Ruggiero

  • C. E. Tanner

Journal or Work Title
  • Applied Optics

Volume
  • 58

Issue
  • 4

First Page
  • 1121

Last Page
  • 1127

ISSN
  • 1559-128X

Publication Date
  • 2019-02

Subject
  • Titan TEM

Date Created
  • 2019-08-07

Language
  • English

Departments and Units
Record Visibility and Access Public
Content License
  • All rights reserved

Digital Object Identifier

doi:10.1364/AO,58,001121

This DOI is the best way to cite this article.