Development of a yeast-based sensor for environmental monitoring

Doctoral Dissertation

Abstract

Part 1: MTBindingSim

MTBindingSim is a program that simulates binding curves for proteins binding to microtubules. The operation of the program, the experimental methods it simulates, and the protein binding mechanisms it models are described. Experimental data are used to demonstrate the utility of MTBindingSim for experimental design and data interpretation. The use of MTBindingSim as an educational tool is discussed, with the result that students found the program useful but were confused by its terminology. This result led to the creation of BindingTutor, a similar program that models general protein-ligand binding.

Part 2: Yeast-based Biosensors for Environmental Monitoring

The development of small continuous culture devices for growing yeast and their application as environmental sensors is described. The systems can be used to detect toxins, phosphate, and mutagens. The development of a prototype, field-deployable sensor and the results of testing it in a lab setting are discussed.

Attributes

Attribute NameValues
URN
  • etd-07182013-184512

Author Julia T Philip
Advisor Dr. Holly Goodson
Contributor Dr. J. Dan Gezelter, Committee Member
Contributor Dr. Jeff Peng, Committee Member
Contributor Dr. Holly Goodson, Committee Chair
Contributor Dr. Rob Nerenberg, Committee Member
Degree Level Doctoral Dissertation
Degree Discipline Chemistry and Biochemistry
Degree Name PhD
Defense Date
  • 2013-07-15

Submission Date 2013-07-18
Country
  • United States of America

Subject
  • phosphate

  • biosensor

Publisher
  • University of Notre Dame

Language
  • English

Record Visibility and Access Public
Content License
  • All rights reserved

Departments and Units

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