Development of a yeast-based sensor for environmental monitoring

Part 1: MTBindingSim

MTBindingSim is a program that simulates binding curves for proteins binding to microtubules. The operation of the program, the experimental methods it simulates, and the protein binding mechanisms it models are described. Experimental data are used to demonstrate the utility of MTBindingSim for experimental design and data interpretation. The use of MTBindingSim as an educational tool is discussed, with the result that students found the program useful but were confused by its terminology. This result led to the creation of BindingTutor, a similar program that models general protein-ligand binding.

Part 2: Yeast-based Biosensors for Environmental Monitoring

The development of small continuous culture devices for growing yeast and their application as environmental sensors is described. The systems can be used to detect toxins, phosphate, and mutagens. The development of a prototype, field-deployable sensor and the results of testing it in a lab setting are discussed.