Kinetics of a Perchlorate-Reducing Bacterium that Accumulates High Levels of Chlorate

Master's Thesis
Thumbnail

Abstract

Perchlorate (ClO4-) is found in potable water sources across the United States. Dissimilatory reduction to chloride by perchlorate-reducing bacteria (PCRB) is an effective treatment strategy, but little is known about the kinetics of PCRB. Two novel PCRB isolates accumulate high levels of chlorate during batch perchlorate reduction. Dechloromonas sp. HCAP-C and Dechloromonas sp. HCAP-1 accumulate chlorate at around 20 % and 13 % of the initial perchlorate concentration, respectively; conventional PCRB accumulate less than 3% chlorate. The kinetic parameters for HCAP-C were determined and found to be distinct from conventional PCRB. HCAP-C had low qmax values (qmax for perchlorate = 4.6 mgVSS/mgVSS-day; qmax for chlorate = 6.5 mgVSS/mgVSS-day) and high K values (K for perchlorate = 40 mg/L; K for chlorate = 20 mg/L). Perchlorate reduction rates appear to increase and chlorate accumulation appears to decrease with co-cultures of HCAP-C with conventional PCRB and chlorate-reducing bacteria.

Attributes

Attribute NameValues
URN
  • etd-07142006-135121

Author Anna Rita Salamone
Advisor Dr. Robert Nerenberg
Contributor Dr. Robert Nerenberg, Committee Chair
Degree Level Master's Thesis
Degree Discipline Civil Engineering and Geological Sciences
Degree Name MSBioeng
Defense Date
  • 2006-07-07

Submission Date 2006-07-14
Country
  • United States of America

Subject
  • Chlorate accumulation

  • Biodegradation

  • Perchlorate

Publisher
  • University of Notre Dame

Language
  • English

Record Visibility and Access Public
Content License
  • All rights reserved

Departments and Units

Files

Please Note: You may encounter a delay before a download begins. Large or infrequently accessed files can take several minutes to retrieve from our archival storage system.